RNA imaging platform for drug discovery and disease detection
Detection of mRNA within cells is a major challenge and is extremely useful in drug discovery. It may also be an important early indicator of major diseases, such as cancer, since mRNA production of cells impacted by disease can exhibit abnormal patterns of mRNA activity. This invention is a platform for the simultaneous detection of thousands of mRNAs within single cells with high efficiency and low error rate. It consists of a nucleic acid probe (composition of matter claims) and a set of method claims for massively parallel probe synthesis and method claims to determine the population of mRNA that are active within the cell at any given time.
A major benefit is dramatically improved throughput: while the predominant existing method (single-molecule fluorescent in situ hybridization or smFISH) can study a few genes at a time, this method allows for the simultaneous detection of all of the thousands of unique RNA species expressed in a cell.
Intellectual Property Status: Patent(s) Pending