Corticospinal tract (CST)-YFP transgenic mice for monitoring axonal responses to spinal cord injury

Hemizygous transgenic mouse strain B6.Cg-Tg(Thy1-EYFP)15Jrs/J (also known as Thy-STOP-YFP) was developed by Dr. Joshua R. Sanes at Harvard University. The mouse strain harbors an Enhanced Yellow Fluorescent Protein (EYFP) controlled by mouse thymus cell antigen 1 theta (Thy1) promoter. In addition, there is a loxP sites flanking STOP codon inserted between the EYFP gene and its photometer. Thus when crossed to a mouse strain expressing Cre recombinase, Thy-STOP-YFP mice will conditionally express EYFP in motor neurons. The hymizygous mice are viable, fertile, normal in size and do not display any abnormalities.

The mouse strain can be used as a tool in studies that require visualization of motor neurons.

The detailed description of the mouse strain can be found here.

The mouse strain is available for licensing from Harvard University and will be provided by The Jackson Laboratory.