The mouse strain 129S-Trpa1tm2Kykw/J was developed by Kelvin Kwan in the laboratory of HHMI Investigator David Corey at Harvard Medical School. Trpa1 conditional knockout animals were generated by flanking the exons that code for the pore domain of the ion channel by loxP sites and selected by presence of a drug resistance cassette. The selection cassette was subsequently removed to produce the Trpa1 conditional knockout allele. The conditional knockout animals serve as a useful tool for cell-type specific ablation of experiments when used in conjunction with Cre/loxP technology.
The detailed description of the mouse strain can be found here.
The mice can be used to model the following conditions:
- Temperature sensitivity
The mouse strain is available for licensing from Harvard University and will be provided by The Jackson Laboratory.