HEK293T cells stably express Divalent Metal Transporter-1 for use in a model system to screen for inhibitors of iron uptake
This invention is a cell-based screen that enables the rapid identification of iron transport inhibitors. From initial efforts, 10 small molecule inhibitors of transport have been identified, and ongoing studies focus on characterizing their mechanisms of inhibition.
Intellectual Property Status: Patent(s) Pending
DMT1 functions in transport of ferrous iron across the plasma membrane and/or out of the endosomal compartment. Thus, the cell line is an ideal tool to screen for potential iron transport inhibitors. In one example, the cell line was used to screen two bioactives libraries for DMT-1 iron uptake inhibitors. Several potential hits were found, including one that may be specific for the DMT1 iron uptake pathway.