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Displaying: 51 - 60 of 103 Results
Modular conditional knockout: Precise temporal control of protein degradation in living cells
Polyubiquitinated proteins in eukaryotes are directed to the proteasome for degradation. It is generally accepted that proteasome-mediated proteolysis depends strictly upon ubiquitination, as disruption of that process has been observed to halt…
DBD
- Maryanne Fenerjian
Investigators
- George Church
- Daniel Janse
Enhanced fidelity of sub-nanogram amplification
The importance of nucleic acid amplification from trace amounts of biological sample has increased with the expansion of genomic sequencing in various clinical and research-oriented applications. This invention characterizes techniques to reduce…
DBD
- Maryanne Fenerjian
Investigators
- George Church
- Kun Zhang
Small-molecule-triggered intein splicing as a universal switch for protein activation
Researchers in the laboratory of Professor David Liu have used directed evolution techniques to evolve an intein-based molecular switch that transduces binding of a small molecule into the activation of an arbitrary protein of interest. To create…
DBD
- Irit Ben-Chelouche
Investigators
- David Liu
- Allen Buskirk
- Sun Peck
A transgenic reporter system can be used for recombination-mediated modulation of gene activity in the developing mouse embryo
A transgenic reporter system consisting of a tamoxifen-inducible form of Cre recombinase (Cre-ERTM) that can be used for recombination-mediated modulation of gene activity in the developing mouse embryo. Using the enhancer of the Wnt1 gene to…
DBD
- Vivian Berlin
Investigators
- Andrew McMahon
- Paul Danielian
- David Rowitch
Transgenic mice with fluorescently tagged neuronal mitochondria as a new tool to study axonal transport
Homozygous transgenic mouse stain B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J (also know as Thy-CFP-MitoS) was developed by Dr. Jeffrey W. Lichtman at Harvard University. The mice express mitochondria-targeting Cyan Fluorescent Protein (CFP) in neuronal cell…
DBD
- Yelena Bisharyan
Investigators
- Jeffrey Lichtman
- Florence Bareyre
- Thomas Misgeld
- Martin Kerschensteiner
HEK293T cells stably express Divalent Metal Transporter-1 for use in a model system to screen for inhibitors of iron uptake
This invention is a cell-based screen that enables the rapid identification of iron transport inhibitors. From initial efforts, 10 small molecule inhibitors of transport have been identified, and ongoing studies focus on characterizing their…
DBD
- Grant Zimmermann
Investigators
- Marianne Wessling-Resnick
- Peter Buckett
Homozygous IKKepsilon knockout mouse as an animal model for influenza virus infection
Homozygous IKKepsilon (inhibitor of kappaB kinase epsilon) mutant mice are used as animal models for influenza virus infection. Homozygous deletion results in a complete loss of the endogenous kinase function in lung, spleen, and embryonic…
DBD
- Grant Zimmermann
Investigators
- Thomas Maniatis
- Benjamin tenOever
Corticospinal tract (CST)-YFP transgenic mice for monitoring axonal responses to spinal cord injury
Hemizygous transgenic mouse strain B6.Cg-Tg(Thy1-EYFP)15Jrs/J (also known as Thy-STOP-YFP) was developed by Dr. Joshua R. Sanes at Harvard University. The mouse strain harbors an Enhanced Yellow Fluorescent Protein (EYFP) controlled by mouse thymus…
DBD
- Yelena Bisharyan
Investigators
- Joshua Sanes
- Joshua Sanes
- Florence Bareyre
Mass spectrometry-based in vitro kinome activity assay
This technology is a new generation, high-throughput assay to quantitatively assess the activity of the kinome in a variety of samples. Cocktails of synthetic peptides are combined with a sample of interest and phosphorylated by kinases in the…
DBD
- Grant Zimmermann
Investigators
- Steven Gygi
- Judit Villen
- Kazuishi Kubota
- Yong Yu
In vitro models and method of treatment for amyotrophic lateral sclerosis (ALS)
It has been impossible to isolate viable human motor neurons from patients or from postmortem samples to investigate the mechanisms leading to neural degeneration in ALS. A potential solution is to use embryonic stem cells (ESC) as a renewable…
DBD
- Vivian Berlin
Investigators
- Kevin Eggan
- Francesco Di Giorgio